Changes in the expression of Toll-like receptors, cytokines and chemokines in the cells of the mucous membrane of the oropharynx in children with COVID-19

Introduction. COVID-19 is a severe respiratory disease caused by the ?-coronavirus SARS-CoV-2, which characterized immune-mediated tissue and organ damage. It has been proven that in children relatively easy compared to adults, mechanisms of innate immunity play an important role this. The aim investigation was study expression genes encoding receptors TLR4, TLR7, as well analyze changes proinflammatory cytokines – interleukin(IL)-1?, tumor necrosis factor (TNF)-? interferon (IFN)-?–in with COVID-19, depending on severity disease. Material methods. included 55 confirmed diagnosis COVID-19. Patient groups were formed basis complaints clinical manifestations. 1st group asymptomatic 9 people. 2nd mild form course 36 people, 3rd consisted moderate (10 people). comparison 25 healthy children. RNA isolated from obtained material level gene determined polymerase chain reaction real time, supernatant method multiplex immunofluorescence analysis. Results. A statistically significant increase for (TLR4, TLR7) cells mucous membrane oropharynx shown. TLR4 TLR7 was, respectively, 2.6 7.2 times higher group. At same pro-inflammatory (IL-1?, TNF?, IFN-?) at protein levels, associated revealed. Our also showed production chemokines, correlated Discussion. An oropharyngeal mucosa indicates their pathogenesis main function recognize pathogen-associated molecular patterns, namely, spike glycoprotein involved penetration virus into target single-stranded virus. Activation leads Toll-induced we have demonstrated. This plays huge development inflammatory response membranes Conclusion. data us can be used further determination genetic markers children, will allow select optimal therapy option future.